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The Rye Laboratory

Department of Biochemistry and Biophysics

Research

About Research in the Rye Lab

We are broadly interested in understanding how the conformational states of proteins and cellular membranes are monitored and controlled inside a living cell.  Using bacteria and yeast as model systems, we employ a variety of methods, including genetics, protein mutagenesis, fluorescence spectroscopy, stopped-flow kinetics and single-molecule fluorescence burst analysis.  Projects in the lab cluster into two thematic areas involving (1) protein dynamics and molecular chaperone function and (2) membrane dynamics.

Current Projects

  • Development of single particle analysis methods
  • Protein disaggregation by molecular chaperones
  • Intracellular protein folding by GroELS
  • Secretory vesicle formation and membrane dynamics

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